Glucose Test
Patient Profiles:
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Procedures:
- Create a data table(see below) to record the color of the control and each patient’s urine sample before heating and the color of each sample after heating. Label it “Chemical Properties of Urine Analysis: Glucose.”
- Add 200 mL of water to a beaker and place on a hot plate to heat. Do not boil the water.
- Place four test tubes in a test tube rack.
- Label one test tube “control” and the others “A,” “B,” and “C.”
- Use the transfer pipette to add 10 drops of Benedict’s solution to each test tube.
- Use a clean transfer pipette for each urine sample and add 3 mL of the urine sample to the appropriate test tube. Record the color of the urine sample in your data table before heating.
- Place each test tube into the hot water bath for 2 minutes.
- Record the color change in your data table. A color change to orange, red, or brown indicates the presence of sugar.
- Clean the work area with surface disinfectant. Remove goggles,